Intrinsic Neuronal Excitability: A Role in Homeostasis and Disease

The neuronal components of brain circuitry are generally considered “stable” throughout an animals’ life; with the exception of times of growth and degeneration that occur during development, aging, or pathology. To maintain this stability, neurons must achieve a balance between changing output to meet new requirements, and keeping output within a satisfactory operating range. This balancing act is done through the combination of synaptic plasticity and changes in intrinsic neuronal excitability. While synaptic plasticity has been the focus of most research into neuronal excitability, growing evidence including that described in the recent review by Beraneck and Idoux (2012), highlights the fact that changes in intrinsic membrane properties also shape the output of single neurons. In their article Beraneck and Idoux (2012) provide evidence for changes in intrinsic excitability of second order vestibular neurons that occur during vestibular compensation – a remarkable example of homeostatic plasticity whereby spontaneous discharge of neurons in the ipsi and contralesional vestibular nuclei is recalibrated following unilateral vestibular damage or labyrinthectomy. While the authors describe clearly how the output of second order vestibular neurons changes with respect to action potential shape and discharge dynamics within the context of vestibular compensation, it is worth noting that the underlying mechanisms of these changes are not isolated to the vestibular system, but appear to be a common feature of homeostatic plasticity in other central nervous system (CNS) structures and during disease. In the following commentary some of the findings highlighted by Beraneck and Idoux are discussed with respect to work on neuronal excitability in other CNS regions. Beraneck and Idoux (2012) detail evidence to support the hypothesis that changes in background discharge during vestibular compensation of vestibular nucleus neurons appears to be predominately expressed as changes in the excitability of the type B medial vestibular nucleus (MVN) neuron subpopulation. Broadly speaking the changes described in their review can be characterized as those that affect discharge rate (e.g., spike threshold, action potential shape), or those that affect discharge pattern. Importantly, similar modifications of intrinsic membrane properties have also been shown in various CNS pathologies, highlighting their importance as potential therapeutic targets during disease. In the commonly used Pilocarpine model of epilepsy where injection of the muscarinic agonist causes seizures similar to those observed in human temporal lobe epilepsy (Sanabria et al., 2001), downregulation of the slow, non-inactivation voltage-dependent potassium current (Ih) has been observed in pyramidal neurons of the hippocampus (Jung et al., 2007), as well as entorhinal cortex (Shah et al., 2004). In these structures attenuation of I h results in hyperpolarization of the resting membrane potential, increased cell resistivity (making the cell more sensitive to small voltage fluctuations), and increased EPSP summation (Shah et al., 2004; Jung et al., 2007). Similarly, changes in the number of voltage-gated sodium (Na+) channels have been demonstrated in animal models of chronic pain. In this example, the threshold required to elicit an action potential from dorsal root ganglion (DRG) neurons was reduced following up-regulated expression of voltagegated Na+ channels (Hong et al., 2004; Kirita et al., 2007; Wang et al., 2007). In addition, down-regulation of somatic I A has been shown in DRG neurons following crush injury of the nerve root (Hu and Gereau, 2003; Hu et al., 2006; Karim et al., 2006; Tan et al., 2006). These changes in DRG neuron intrinsic excitability, presumably underlie the hyperalgesia and allodynia commonly observed in chronic pain models. While the changes in intrinsic excitability described above can be broadly characterized as those that affect the properties of all-or-none action potentials, experience-dependent changes can also impact on the pattern of neuronal discharge, that is, the number or regularity of action potentials. Beraneck and Idoux (2012) discuss a homeostatic shift toward the phasic type B-like discharge phenotype (including the subset of neurons with lowthreshold “burst” spikes) following chronic attenuation of inhibitory drive, and on the contralesional side following vestibular compensation. Importantly, this change in discharge phenotype is also observed in a number of pathophysiological states (Beck and Yaari, 2008). For example, changes in firing mode toward a phasic phenotype have been reported in models of chronic epilepsy (Sanabria et al., 2001), and in other epilepsy-associated disorders (Baraban and Schwartzkroin, 1995). Further, increases in the number of neurons displaying the burstfiring phenotype have been implicated in other CNS disorders including chronic stress (Okuhara and Beck, 1998), pain (for review see Cummins et al., 2007; Hains and Waxman, 2007), and neuroinflammation (Saab et al., 2004). What underlies experience-dependent changes in firing mode? In burst-firing neurons such as the phasic type B plus lowthreshold spikes MVN neuron population, a subthreshold depolarization (termed an afterdepolarization; ADP) can exceed action Intrinsic neuronal excitability: a role in homeostasis and disease